Primary human CLL cells have high levels of Par-4 protein expression. (A) Immunoblot analysis of activated SFK and Par-4 protein expression levels in primary human CLL (hCLL) samples compared with the whole peripheral blood lysate of normal donors (WBC). Protein values are normalized to β-actin. (B) Cell lysates of purified B cells from CLL patients (n = 4) and from healthy donors (n = 4) were probed for Par-4. The bar graph represents Par-4 protein values normalized to GAPDH expression. P = .022 determined by Student t test. (C) Survival curves of hCLL cells treated with BCR-signaling inhibitors. Assay performed in triplicate. Error bars represent SEM. (D) Primary human peripheral blood CLL cells (97.7% CD5⁺CD19⁺) were treated with 1 μM dasatinib (left) and 5 μM fostamatinib (right). p-SFK and Syk levels are normalized to their respective total protein levels. Total Syk, Lyn, and Par-4 protein expression levels are normalized to β-actin.