DIAPH1, DIAPH2 and DIAPH3 expression during MK differentiation. (A) CD41⁺ MKs were sorted on day 6 of culture and recultured for various periods according to the experiments. In the western blots, HSC70 was used as the protein loading control; an 8% SDS-PAGE gel was used for protein separation. (B) The ratio between DIAPH (DIAPH1, DIAPH2, or DIAPH3) and HSC70 protein was analyzed by Image J in 3 independent experiments. DIAPH1 expression was increased, and DIAPH2 and DIAPH3 were decreased during MK differentiation. (C) RT-PCR (normalized to HPRT) was performed on CD41⁺ MKs collected at days (D)7, D9, and D12 of culture (3 independent experiments). DIAPH1 mRNA expression was increased during MK differentiation. *P < .05; **P < .005.