Inhibitory effects of FF-10101 on colony formation of human mononuclear cells. (A-D) Mononuclear cells isolated from 4 independent CB samples were placed in methylcellulose semisolid medium with increasing concentrations of FLT3 inhibitors (n = 3) and incubated at 37°C for 10 (panel A-B) or 14 days (panel C-D). After incubation, CFU-GM, CFU-GEMM, and BFU-E colonies were manually counted. Mean colony numbers ± SD in each colony are shown.