Suppression of elevated mTORC1 activity by rapamycin attenuates IE of iron-deficient Hri^−/−, eAA, and Atf4^−/−mice. (A) CBC analysis of blood samples after 12 days of vehicle or rapamycin (Rapa) treatments. (B) Spleen weights as percentage of body weight. (C) Serum Epo levels. P values denote the comparison between vehicle and rapamycin treatment of each genotype. *P < .05, **P < .01, ***P < .001. (D) Representative blood smears before (day 0) and after 12 days (day 12) of rapamycin treatments. Arrows indicate globin inclusions. (E) Erythroid differentiation of BM and spleen. (F) mTORC1 activity in total BM and spleen cells. Numbers of mice used in (A-C and E), +Fe conditions: Wt, n = 3; Wt+rapa, n = 3; –Fe conditions: Wt, n = 5; Wt+rapa, n = 4; Hri^−/−, n = 4; Hri^−/−+rapa, n = 5; eAA, n = 4; eAA+rapa, n = 5.