Long-term incubation of fibrinogen/fragment D with Aβ42 forms a SDS-stable complex. (A) Aβ42 G37D or Aβ42 was incubated with fragment D (FD) for 5 days at 37°C in the presence or absence of EDTA. Western blots were analyzed with antibody 6E10 against Aβ (left panel) and an antibody against fibrinogen (Dako; right panel). Aβ-fragment D SDS-stable complex was detected by 6E10. (B) Aβ42 G37D was incubated with fibrinogen (FBG) for 24 hours at 37°C. Western blots were analyzed with antibody 6E10 against Aβ (left panel) and an antibody against fibrinogen (right panel). Aβ-fibrinogen SDS-stable complex was also detected by 6E10 (arrow). Controls and other lanes in panel B are from the same gel with some lanes omitted for clarity.