Figure 2
In vitro thrombus formation of SERCA3−/− platelets compared with controls. Rhodamine 6G–labeled platelets were injected into capillaries precoated with collagen (50 μg/mL) at low (150 s−1; A) or at high (1200 s−1; C) shear rates in absence or in presence of both apyrase (5 U/mL) and indomethacin (5 µM), noted “Apy-Indo.” Images show platelet adhesion and thrombus formation after 3 minutes of perfusion, and plots (B, D) represent the quantification of platelet adhesion, expressed as covered surface area relative to controls given as 100%. Absence or presence of Apy-Indo is noted “-” or “+” below plots. Data were calculated using 1-way ANOVA followed by Tukey’s multiple comparison test; ns, not significant; ***P < .001.
