Phagocytosis of anti-RhD opsonized erythrocytes by monocyte-derived macrophages. (A) RhD-positive erythrocytes opsonized with anti-RhD after phagocytosis by monocyte-derived macrophages cultured for 9 days, before (left) and after (right) lysis of erythrocytes. (B) Representative images of the quantification of phagocytosis by flow cytometry. Unopsonized or anti-RhD opsonized CFSE-labeled RhD-positive erythrocytes were incubated with macrophages in the presence or absence of cytochalasin B. Percentages indicated the percentage CFSE-positive macrophages relative to the total macrophage population. Data are representative of at least 6 (cytochalasin B) experiments. (C) Time course of phagocytosis of anti-RhD opsonized erythrocytes by M-CSF and GM-CSF macrophages. Data are representative of 3 independent experiments. Error bars represent standard error of the mean. (D) Comparison of IgG-mediated phagocytosis (corrected for unopsonized phagocytosis) for M-CSF and GM-CSF macrophages (note different points). Mean and dots represent experiments with macrophages of different individuals (n = 58 for M-CSF, n = 55 for GM-CSF). Some individuals were measured twice; the mean of the measurements is shown for these individuals. Statistics with Mann-Whitney test.