FcγR expression on monocyte-derived macrophages. (A) Histograms showing expression levels of different FcγRs in monocyte-derived macrophages cultured for 9 days with M-CSF. Gray shading: relevant isotype control (B-E) comparison of expression levels of FcγRI, FcγRIIa,b, FcγRIIb, and FcγRIII between M-CSF and GM-CSF macrophages. Data represent the mean of experiments with macrophages from 34 individuals, or 22 independent experiments in the case of FcγRII (including only individuals without an FCGR2C-open reading frame). Some individuals were measured twice; means of the measurements were used for these individuals. Error bars represent standard error of the mean, statistics with Mann-Whitney test. (F) Percentages of FcγRIII-low and FcγRIII-high macrophages (left, individual experiments are shown) and comparison of the expression levels of the low and high populations (right, data represent the mean and standard error of the mean of at least 12 individuals).