Figure 1
AMIS by individual monoclonal or polyclonal HEL antibodies. Mice were not transfused (Nil) or were transfused with 107 HOD RBCs alone (0 μg) or in the presence of increasing concentrations (0.001, 0.01, 0.1, 1.0, and 5.0 µg) of HEL-specific antibodies. (A) Suppression by monoclonal 8E12. (B) Suppression by monoclonal 4B7. (C) Suppression by monoclonal 5B9. (D) Suppression by monoclonal 2F4. (E) Suppression by polyclonal HEL-specific IgG. Mice were bled for serum on day 0 (1 hour after transfusion as a control) and on day 7. IgM antibodies with specificity for HEL were evaluated by ELISA. Data represent the mean ± SEM of 3 separate experiments.
