HDAC11 regulates the production of cytokines and chemokines in HL. (A) We analyzed 4 cell lines: HD-LM2, L428, KM-H2, and Mino. Cell culture supernatants were collected after 24 hours of HDAC11 siRNA transfection. HDAC11 siRNA increased the production of IL-13, TNF-α, interferon-γ, IL-6, and IL-17. Each value is the mean of 3 independent experiments (±SEM). *P < .05, **P < .005. (B) Expression of OX40L after TNF-α siRNA transfection. The L428 cell line was analyzed by FACS after TNF-α siRNA transfection and after 48 hours of treatment with MGCD0103 (1.0 μM). MGCD0103 increased OX40L expression. (C) OX40L expression in HD-LM2 cells. HD-LM2 cells were incubated with recombinant TNF-α (1-10 ng) for 48 hours and then analyzed by FACS. Recombinant TNF-α did not have a significant effect on OX40L expression in these cells.