CYTH1 knockdown decreases long-term engraftment of CD34⁺ cells in NSG mice and impairs homing to bone marrow. (A) Outline for the in vivo assessment of CD34⁺ cells long-term engraftment capacity (upper). Analysis of human chimerism in mice transplanted with SCR and CYTH1-sh1 transduced cells (lower). Data accumulated from 2 independent experiments. Lineage distribution is shown only for recipients with at least 1% of human engraftment. (B) Outline for the assessment of CD34⁺ cells’ homing capacity. Analysis of human chimerism 18 hours following intravenous injection of transduced cells. Data accumulated from 2 independent experiments.