Platelets are not required for lung neutrophil accumulation or NET formation in the lungs. Neutrophil accumulation in lung tissue was measured by quantification of Ly-6G stainings (A-B) and MPO measured in lung homogenates (C). NETs were stained using antibody to H3Cit for lung (D) and liver (E) and then quantified (F-G). Original magnification ×4 for panels A, D, and E; H&E stain. Ly-6G and NET positivity and total lung surface area were measured using Image J (National Institutes of Health, Bethesda, MD; http://rsb.info.nih.gov/ij), with the amount of Ly-6G or NET positivity expressed as a percentage of the total surface area. Data are expressed as box-and-whisker plots depicting the smallest observation, lower quartile, median, upper quartile, and largest observation. n = 8 mice per group. *P < .05.