NRAS^G12V withdrawal abrogates self-renewal capacity. (A) Colony-forming assay (CFA) schematic. Doxycycline was administered on day 0. Colonies that grew by day 7 were extracted, dispersed, and plated in methylcellulose for a secondary CFA with no further treatment (doxycycline was not administered after day 0). (B) Primary colony formation of primary leukemia cells treated with doxycycline or vehicle on the day of plating. Each bar represents the average value for 13 independent experiments. (C) Secondary colony formation of replated colonies. Each bar represents the average value for 3 independent experiments. Error bars represent standard error of the mean.