NRASG12V/Mll-AF9 AML On-Off model and gene expression. (A) Tumor burden was assayed by peripheral white blood cell (WBC) count as a fraction of the WBC count of each mouse at the time of sacrifice to the WBC count at the time of doxycycline treatment. Each point represents the mean WBC fraction of all of the animals sacrificed at that time point. Error bars represent the standard error of the mean for each time point. (B) NRASG12V transgene abundance by quantitative polymerase chain reaction. (C) NRASG12V protein levels by Western blot analysis. (D) Hierarchical clustering of gene-expression microarray and RNA sequencing data using the list of differentially expressed genes identified by microarray analysis. (E) Ingenuity Upstream Regulator Analysis identified transcriptional programs that were repressed (negative Z score, top panel) or mimicked (positive Z score, bottom panel) by NRASG12V withdrawal.
