In DLBCL, IL-10, PD-L1, and S100A9/12 are expressed by monocytes. (A) Correlation of the T_reg and M-MDSC counts in DLBCL peripheral blood (n = 17). (B) Expression of the immunomodulatory genes IL10, TGFβ1, CD274, S100A8, S100A9, and S100A12 evaluated by TLDA. The relative gene expression (mRNA) of PBMC from DLBCL and HD was compared (ratio of the mean expression on DLBCL to the mean expression on HD; n = 17 DLBCL and 15 HD). (C) Surface PD-L1 by flow cytometry expressed as ratio of mean fluorescence intensity for CD14^posHLA-DR^low to CD14^posHLA-DR^high (n = 13 DLBCL and 8 HD). (D) Cytokines detection by ELISA in HD (n = 8) and DLBCL plasma: IL-10 (n = 45), TGF-β (n = 14), S100A8/9 (n = 14), and S100A12 (n = 14). (E) Cytokine (IL-10 and S100A12) detection by ELISA in supernatants of PBMCs not depleted (+) or depleted (−) in CD14^pos cells (n = 13 DLBCL). *P < .05, **P < .01, ***P < .001.