hTERT overexpression increases telomerase activity and induces apoptosis. (A) Telomerase activity with overexpression of hTERT variants. Error bars show standard deviation of biological triplicates. (B) RNA levels with overexpression of hTERT variants. Error bars show standard deviation of PCR triplicates. (C) Live cell counts with overexpression of hTERT variants. Error bars represent standard error of the mean of biological triplicates. (D) Apoptosis measured by caspase-3/7. Error bars show standard deviation of biological triplicates. *P < .05 assessed by 1-way ANOVA.