UHRA-10 inhibits thrombus formation in mouse cremaster arterioles in a dose-dependent manner. (A-E) Binarized images from 1 representative injury each showing the effects on accumulation of platelets (red) and fibrin (green) 120 seconds after laser-induced injury to the vessel wall in mice administered either (A) saline or UHRA-10 at (B) 10 mg/kg, (C) 20 mg/kg, (D) 40 mg/kg, or (E) 80 mg/kg. Scale bars: 10 μm. (F-I) Statistical analyses of the dose-dependent attenuation of thrombus formation by UHRA-10; data are from 27 to 30 injuries to 5 mice for each condition. Median integrated fluorescence intensities (nonbinarized) were plotted vs time for accumulation of (F) platelets and (H) fibrin. In addition, the area under the curve (total fluorescence intensity) for each individual injury was plotted for accumulation of (G) platelets and (I) fibrin (each point represents 1 injury, plotted as log value). Note: data for saline control and UHRA at 40 mg/kg are in common with the data from Figure 1 and are therefore repeated here in (A) and (D), and the blue lines and data points in (F-I). Median values were compared with saline control for statistical significance by Mann-Whitney U test. UHRA-10 significantly reduced platelet accumulation at doses of 20 and 40 mg/kg and significantly reduced fibrin accumulation at doses of 40 and 80 mg/kg. *P < .05; **P < .005. Data were captured and analyzed as in Figure 1.