The AA phenotype in ARE-del mice is not a result of a cell nonautonomous effect of IFN-γ on the BM microenvironment. (A) The function of stromal cells in ARE-del mice was determined by the serum levels of Flt3L, SCF, IL-3, IL-6, and EPO. The concentrations of cytokines were measured by using an enzyme-linked immunosorbent assay and shown in bar graphs (n = 6). BM phenotypes in WT>WT and ARE-del >WT mice were analyzed 8 weeks after reconstitution. (B) The bar graph shows BM cell numbers of chimeric mice (n = 6). (C) HSPC composition was analyzed on lineage-negative BM cells from WT>WT and WT>ARE-del mice by using flow cytometry. Density plots are representative of HSPC gating from 1 set of 3 experiments. (D) Total BM cells from WT>WT and WT>ARE-del mice were stained with fluorochrome-conjugated antibodies for lineage differentiation analysis. Dot plots are representative of 1 set of 3 experiments. Similar results were obtained from 3 different experiments. Results are expressed as mean ± SD. *P < .05; **P < .01; ****P < .0001.