Constant exposure to IFN-γ interrupts RBC and B-cell differentiations. Total BM cells were stained with fluorochrome-conjugated antibodies for lineage differentiation analysis. (A) Different developmental stages of RBCs were gated according to the expression levels of Ter119 and CD71 (R1: proerythroblasts, Ter119^medCD71^high; R2: early basophilic erythroblasts, Ter119^highCD71^high; R3: polychromatophilic erythroblasts, Ter119^highCD71^med; R4: orthochromatophilic erythroblasts, Ter119^highCD71^lo). ProE cells are gated on the CD71^hi and Ter119^low population. Ter119^hi population was then further defined on the basis of the expression levels of CD71 and cell size into EryA (CD71^highFSC^high), EryB (CD71^highFSC^low), and EryC (CD71^lowFSC^low). (B) Live IgM^–B220⁺ population in BM was measured by using CD43 and CD19 to define PreProB (CD43⁺CD19^–). PreB (B200⁺CD43^–) and ProB (B220⁺CD43⁺) populations were gated on live IgM^– population in BM using CD43 and B220. Mature B cells were gated on a live IgM⁺B220⁺ population. (C) Myeloid cells in BM were gated on the Mac1⁺CD11b⁺ population. Dot plots are representative of 1 set of multiple experiments. Bar graphs show the percentage of total live BM cells and the total cell numbers of each cell type (n = 5). Similar results were obtained from 4 different experiments. Results are expressed as mean ± SD. *P < .05; **P < .01; ***P < .001; ****P < .0001.