IFN-γ and IFN-γ signaling are detected in ARE-del BM. (A) T cells were gated on the live NKp46^–CD11b^–B220^–Gr1^–CD3⁺ population, and NK cells were gated on the live NKp46⁺CD11b^–B220^–Gr1^–CD3^– population. IFN-γ expression by BM NK and T cells was detected by using intracellular cytokine staining. Dot plots are representative of 1 set of 4 experiments. (B) Total BM cells from WT and ARE-del mice were sorted into LSK (LT-HSCs, ST-HSCs, and MPPs) and LK (CMPs, GMPs, and MEPs) populations and centrifuged onto slides. The cells were then stained with antibody against IFN-γ receptor. The expression of IFN-γ receptor was captured with a Zeiss LSM 710 confocal microscope. (C) Total protein was extracted from untreated WT and ARE-del BM. Proteins were detected by western blotting with antibody against phosphorylated Stat1 (pSTAT1) tyrosine 701 residue. Stat1 and actin were used as loading controls. Positive control (PC) is protein extracted from WT splenocytes stimulated with IFN-γ for 15 minutes. DAPI, 4,6 diamidino-2-phenylindole. FSC, forward side scatter.