RXR loss induces PML/RARA detachment from target genes. (A) APL mice were treated or not for 48 hours ex vivo with 4-OHT. Immunoprecipitated DNA with the indicated antibodies was analyzed by qPCR as indicated. Error bars represent standard deviations of 3 independent biological replicates, and significance was assessed using Student t test. *P < .05; **P < .01. (B) Comparison of gene expression activation (left; 1.7-fold up) or repression (right; twofold down) after 16 hours of ex vivo treatment with 4-OHT (red) or RA (blue). See supplementary Figure 1 for primary data. (C) Tgm2 gene expression after 16 hours of ex vivo treatment with 4-OHT or RA (2 biological replicates).