BCL-2 expression and ABT-199 sensitivity in the Lck-Lmo2 transgenic mouse model. (A) ABT-199 response of tumor cells obtained from Lck-Lmo2 transgenic mice. T-cell tumor #39365 consists of approximately 90% double-negative T-cells (approximately 40% double-negative 1, 60% double-negative 2), whereas tumor #39354 consists of about 30% double-positive and 40% CD4⁺ single-positive T-cells. The viability (average and standard deviation of 2 independent experiments) is shown relative to the cells incubated with dimethylsulfoxide (100%). Based on these data, the IC₅₀ values were calculated. (B) BCL-2 expression in the Lck-LMo2 mouse tumors. The top panel shows the mRNA expression (quantitative real-time polymerase chain reactions, calibrated normalized relative quantities values and corresponding standard errors) and the bottom panel shows the protein expression with β-actin as loading control. The messenger RNA gene expression was standardized against Hmbs, Tbp, and Ubc.