GPR84 confers a growth advantage on KLSA9M pre-LSCs. (A) Western blot analysis of GPR84 and β-catenin expression in KLSA9M pre-LSCs transduced with GPR84 cDNA or EV, as well as KLSMLL pre-LSCs transduced with EV. (B) Colony-forming assays of KLSA9M pre-LSCs expressing GPR84 cDNA or EV (N = 3). (C) Kaplan-Meier survival curves for mice that received 1 × 10⁶ KLSMLL pre-LSCs expressing EV, KLSA9M pre-LSCs expressing GPR84 cDNA, or EV. (D) Representative flow cytometry plots illustrating in vivo short-term cell proliferation assays of GFP⁺ KLSA9M pre-LSCs expressing GPR84 or EV 10 days after transplantation (N = 3 independent experiments). BM cells isolated from normal mice were used as a GFP-negative control to set up an appropriate flow cytometric gate.