Expression of constitutively active β-catenin rescues the GPR84-deficient phenotype in vitro and in vivo. Scr or GPR84 KD#1 KLSMLL GFP⁺ leukemic cells were sorted from BM cells of primary recipients and subsequently transduced with constitutively active β-catenin (βcat*) or empty vector (EV). (A) Western blot analysis of β-catenin expression in transduced GFP⁺ KLSMLL leukemic cells. (B) Colony-forming assays of transduced GFP⁺ KLSMLL leukemic cells. NS, not significant (P > .05). Mean of assay performed in triplicate. (C) Kaplan-Meier survival curves for secondary recipient mice that received 1 × 10⁵ transduced GFP⁺ KLSMLL leukemic cells.