Analysis of Stat3 and Stat5 phosphorylation in BM and spleen of transgenic mice (n = 4, per group). Intracellular detection by flow cytometry of pStat3 (A) and pStat5 (B) in BM and spleen. Data shown are representative of 2 independent experiments (n = 4 per arm). (C) Levels of total Stat3 and pStat3 proteins (left panel), and Stat5 and pStat5 proteins (right panel) in BM homogenates assessed by immunoblot analysis. Error bars represent SEM. One-way ANOVA is shown for comparisons. MFI, mean fluorescent intensity; ns, not significant.