Figure 3
Flow cytometric analysis of BM and spleen, and hematopoietic progenitor colony assays. (A) flow cytometric analysis of LSK in BM and spleen and quantification of LSK, (B) flow cytometric analysis of erythroid precursors in region II (CD71+/Ter119+) and (D) flow cytometric analysis of megakaryocytic progenitors (Lin−Sca1−ckit+/CD150+CD41+). (C,E) Number of hematopoietic progenitors assessed by colony assays in methylcellulose or collagen-based media. Error bars represent SEM. One-way ANOVA was used for comparisons. *P ≤ .05; ns, not significant. (SclCreER;V617F;Stat1+/+, n = 5; SclCreER;V617F;Stat1+/−, n = 8 ; SclCreER;V617F;Stat1−/−, n = 9; wild type, n = 8; and Stat1−/−, n = 6). AchE, acetylcholinesterase; FCS, forward scatter; SSC, side scatter.

Flow cytometric analysis of BM and spleen, and hematopoietic progenitor colony assays. (A) flow cytometric analysis of LSK in BM and spleen and quantification of LSK, (B) flow cytometric analysis of erythroid precursors in region II (CD71+/Ter119+) and (D) flow cytometric analysis of megakaryocytic progenitors (Lin−Sca1−ckit+/CD150+CD41+). (C,E) Number of hematopoietic progenitors assessed by colony assays in methylcellulose or collagen-based media. Error bars represent SEM. One-way ANOVA was used for comparisons. *P ≤ .05; ns, not significant. (SclCreER;V617F;Stat1+/+, n = 5; SclCreER;V617F;Stat1+/−, n = 8 ; SclCreER;V617F;Stat1−/−, n = 9; wild type, n = 8; and Stat1−/−, n = 6). AchE, acetylcholinesterase; FCS, forward scatter; SSC, side scatter.

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