The 5′UTR of CARD11 represses protein translation. SUDHL4 and OCI-Ly10 cells were harvested 24 hours after treatment with Isotype + Vehicle, Isotype + 10 nM Silvestrol, anti-BCR + Vehicle, or anti-BCR + 10 nM Silvestrol. (A) Protein levels of CARD11, BCL10, and MALT1 were measured by western blotting. GAPDH served as a loading control, and data are representative of 2 independent experiments. (B) Cells were treated with 100 μg/mL of cycloheximide, and lysates were collected at the indicated time points. Protein levels of CARD11, BCL10, and MALT1 were measured by western blotting. GAPDH served as a loading control, and data are representative of 2 independent experiments. (C) Predicted 5′UTR structures and ΔG of respective genes. (D) A graph of the ratio between Firefly luciferase units (FLU) and renilla luciferase units (RLU) demonstrating the impact each respective 5′UTR has on cap-dependent translation. Data are means ± SEM from triplicates and are representative of 3 different experiments. (E) A graph of the fold change from Isotype + Vehicle for CARD11, BCL10, and MALT1 5′UTR FLU/RLU ratios normalized to GAPDH’s 5′UTR FLU/RLU ratio. Data are means ± SEM from triplicates and are representative of 3 different experiments (*P < .05).