Validation of protein targets identified by LC-MS/MS. Ex vivo monocytes were treated with 200 µg/mL of individual IgG samples (10 VT or 10 HC) for 6 hours and levels of mRNA measured by qPCR (A-D). Data points represent the fold change expression of each sample compared with untreated; mean of duplicates and standard errors are displayed. Data are representative of at least 3 independent experiments. Statistically significant difference (*P < .05) was determined by unpaired Student t test.