Dysregulation of miR-29b and Sp1 expression in Mll^PTD/wt:Flt3^ITD/wtdKI leukemia. Real-time quantitative RT-PCR was performed on 3 to 8 bone marrow samples obtained from leukemic Mll^PTD/wt:Flt3^ITD/wt dKI mice, single mutant Mll^PTD/wt mice, single mutant Flt3^ITD/wt mice, or wild-type control, age-matched mice. (A) pri-miR-29b-2. (B) pri-miR-29b-1. (C) Sp1. (D) Immunoblotting for Dnmt1, 3a, and 3b was performed on whole cell lysates from bone marrow (n = 3 for each genotype). Actin was used as a loading control.