MMP-9 expression was upregulated by Hes1. (A) Real-time RT-PCR for MMP-9 in Hes1- or mock-transduced CMPs and GMPs. (B) Real-time RT-PCR for MMP-9 in Hes1- or mock-transduced 32Dcl3 cells. (C) Real-time RT-PCR for MMP-9 in Hes1-ER– or mock-transduced M1 cells cultured in the presence or absence of 4-hydroxy-tamoxifen (4-OHT) for 60 hours. (D) Real-time RT-PCR for MMP-9 in M1 cells transduced with Hes1, BCR-ABL, BCR-ABL and Hes1, or mock. (A-D) Expression levels were normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. The relative expression level of mock-transduced CMPs (A), mock-transduced 32Dcl3 cells (B), mock-transduced M1 cells cultured in the absence of 4-OHT (C), or mock-transduced M1 cells (D) was defined as 1. All data points correspond to the mean and the standard deviation (SD) of 3 independent experiments.