Figure 4
![Figure 4. Overexpression of DAPK and [K42A]DAPK increases caspase-1 activation and mature IL-1β generation. THP-1 cells were infected with pGC-YFP, pGC-YFP-DAPK, or pGC-YFP-[K42A]DAPK, and YFP-expressing cells isolated by sorting. Control (YFP) and DAPK-expressing THP-1 cells were treated with PMA for 16 hours and then stimulated with MSU (A), or R848 (B) for 6 hours. The IL-1β contents in supernatant were determined by ELISA (C). The amounts of the cleaved IL-1β, procaspase-1, caspase-1 (p10), DAPK, pro-IL-1β, and GAPDH in total cell lysates were determined by immunoblots (A-B). All experiments have been repeated 3 times with similar results. **P < .01, ***P < .001 for paired t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/3/10.1182_blood-2010-08-303115/4/zh89991064580004.jpeg?Expires=1724274562&Signature=n8xT12SFZNVyxUMTyzYED18Uc8wUud1HtT0a0jRyubD8MD799FBNjmIeABL4Nswna5IQSn6bzkljA0SkODG0or0kHpcsI4KgRKzvXvof09TN65kdOi0HfSnActU-~GRCV-YN4icK4bMmplFL3toP8auNHKSP9NI0IzHhNlN8NFqJsaydudFdTKac9pbf6zG3W3pt~RufIIWmiB5s9cTNQwyTT~WCL1NUpY8peYY1dGPPaWhso~rESY4iqY1d~boc3FM3FPPdMIfhQBEqaXuQ1mDVZIPem7J4qQ34oDAnTjs4g3PW4pwsyjwJD7vwbEoyOOWtFYxiXbzL1Ol6-n1Beg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Overexpression of DAPK and [K42A]DAPK increases caspase-1 activation and mature IL-1β generation. THP-1 cells were infected with pGC-YFP, pGC-YFP-DAPK, or pGC-YFP-[K42A]DAPK, and YFP-expressing cells isolated by sorting. Control (YFP) and DAPK-expressing THP-1 cells were treated with PMA for 16 hours and then stimulated with MSU (A), or R848 (B) for 6 hours. The IL-1β contents in supernatant were determined by ELISA (C). The amounts of the cleaved IL-1β, procaspase-1, caspase-1 (p10), DAPK, pro-IL-1β, and GAPDH in total cell lysates were determined by immunoblots (A-B). All experiments have been repeated 3 times with similar results. **P < .01, ***P < .001 for paired t test.
