Figure 5
Figure 5. Effect of RAP-536 on ROS in Hbbth1/th1 mice. Hbbth1/th1 mice (3-4 months old) were treated with RAP-536 or vehicle as in Figure 2, and age-matched wild-type mice treated with vehicle were used as additional controls. (A) Representative flow cytometric gating and quantitative data for ROS obtained by measuring H2DCF-DA fluorescence in immature (CD71+Ter119+) and mature (CD71–Ter119+) erythroblasts from bone marrow and spleen. (B) Representative histogram overlay and quantitative data for ROS in peripheral erythrocytes under basal conditions (without exogenous peroxide) (n = 4-6 mice per group). Data are means ± SEM. ###P < .001 vs wild type; *P < .05, **P < .01, and ***P < .001, RAP-536 vs vehicle.

Effect of RAP-536 on ROS in Hbbth1/th1 mice.Hbbth1/th1 mice (3-4 months old) were treated with RAP-536 or vehicle as in Figure 2, and age-matched wild-type mice treated with vehicle were used as additional controls. (A) Representative flow cytometric gating and quantitative data for ROS obtained by measuring H2DCF-DA fluorescence in immature (CD71+Ter119+) and mature (CD71–Ter119+) erythroblasts from bone marrow and spleen. (B) Representative histogram overlay and quantitative data for ROS in peripheral erythrocytes under basal conditions (without exogenous peroxide) (n = 4-6 mice per group). Data are means ± SEM. ###P < .001 vs wild type; *P < .05, **P < .01, and ***P < .001, RAP-536 vs vehicle.

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