Genomic transcriptional alterations in immature Trim28 mutant erythroid cells. (A) Gene ontology analysis. Genes that exhibit statistically significant differences between control mice and Trim28 mutant mice in the RNA-Seq experiment were analyzed by DAVID bioinformatics resources,³⁷  and the top 10 categories for each list are shown. (B) Fragments per kilobase of exon per million mapped fragments from RNA-Seq for mitophagy and apoptosis-related genes. (C) Apoptosis analysis. Cells were isolated from bone marrow 2 weeks after poly(I:C) treatment, stained with antibodies and FITC-conjugated annexin V, and analyzed by flow cytometry. The frequency of annexin V⁺ DAPI^- early apoptotic cells in each erythroid progenitor population is shown. CD71^hiTER119⁺ or CD71^hiTER119^- cells were further subdivided by forward scatter and CD44 expression. Each circle represents an individual animal, whereas the black bars represent the averages. Data represent a summary from 2 independent experiments. * indicates statistically significant P < .05. NS: not significant, P > .05. (D) Summary of the phenotypes observed in the TMC mutant mouse.