Methylation status of IGSF4 tested by MS-PCR. Figure showing results of MS-PCR in AML patients and cell lines. Three separate regions of the promoter were investigated; region 1, 5, and 9. Each column shows results for a specific primer pair (M, methylated; U, unmethylated). The top of the figure shows the IGSF4 methylation status of several patients (indicated by number), and the bottom shows methylation status of cell lines and M and U control DNA. On the left the identity of each sample is indicated, and on the right the cytogenetic group each patient or cell line belongs to is shown. In patients with t(9;11) (n = 5), no bands are seen with the methylated-specific primers and heavy bands are seen with the unmethylated-specific primers. In contrast, the other MLL patients (n = 5) and other AML patients (n = 5) do show a band with the methylated primer. This difference is also seen in the cell lines.