Skin, stomach, and duodenal histology in AAT-treated and albumin-treated C57/BL6J mice transplanted from C3H.SW-H2^b/SnJ donors. Mice were sacrificed on day 21 after transplantation (3 mice per group). Tissues were placed in 10% formalin, embedded in paraffin, sectioned, stained with H&E, and analyzed. Slides were imaged on an Axio Observer inverted microscope (Carl Zeiss MicroImaging) and captured with an Axiocam MRm camera (Carl Zeiss MicroImaging). (A) Skin section of albumin-treated mouse showing edema with interstitial inflammation (10×). (B) Skin section of albumin-treated mouse showing interstitial fibrosis and inflammation invading and damaging hair follicles, characteristic of GVHD (10× lens). (C-D) Skin of 2 AAT-treated mice showing normal histology (10× and 4×, respectively). (E) Stomach of albumin-treated mouse showing interstitial gastritis and evidence of GVHD. (F) Stomach of albumin-treated mouse showing interstitial gastritis and evidence of GVHD with tissue edema and lymphocytic infiltration. (G) Forestomach of AAT-treated mouse showing minimal evidence of GVHD (10×). (H) Stomach of AAT-treated mouse showing normal histology. (I-J) Glandular forestomach/duodenum of albumin-treated mouse showing interstitial inflammation, crypt loss, and apoptotic bodies with 1 small crypt abscess, typical for GVHD (4× and 10×). (K-L) Duodenum of AAT-treated mouse with minimal focal evidence of GVHD (4× and 10×).