NPM-p30 and NPM-p20 participate in macrophage quiescence. Macrophages were obtained by M-CSF treatment of human peripheral blood monocytes for 4 days, transfected with an empty pcDNA3.1 vector, this vector expressing D213A-mutated NPM1 (MUT), the p30 N-terminal fragment of NPM1 (P30), or the p20 N-terminal fragment of NPM1 (P20). In all panels, NS indicates nonsignificant; **P < .025; ***P < .01; ****P < .001. (A) Left panel, reverse transcription–quantitative PCR of NPM1; control of overexpression. Middle panel, Phagocytosis of fluorescent E coli bioparticles was determined by flow cytometry (mean ± SD of mean fluorescence indexes, triplicates). Right panel, Phagocytosis of apoptotic bodies was determined by flow cytometry (mean ± SD of mean fluorescence indexes, triplicates) 24 hours after transfection. (B) Transwell migration assay; cells were counted in the top panel after 24 hours of migration. (C) Recolonization capabilities were monitored 24 hours later in a wound-healing assay. (Inset) NPM1 protein level was checked by immunoblotting 24 hours after transfection.