Staining of human and murine tissues for nucleolin and calreticulin. Formalin-fixed, paraffin-embedded tissue was stained with Abs to nucleolin (A,C) calreticulin (E,G), or equal concentrations of control IgG (B,D,F,H). Strong endothelial staining for nucleolin can be observed in human tonsil (A) and mouse kidney (C). Endothelial staining for calreticulin can be observed in mouse kidney (E) and brain (G). Photomicrographs were taken at 63×.