Naive CD8⁺ T cells are the most abundant subset of human CD8⁺ T cells. Cryopreserved leukapheresis samples were thawed and rested overnight. (A) Frequency of T_N (CD62L⁺, CD45RO⁻), T_CM (CD62L⁺, CD45RO⁺), and T_EM (CD62L⁻, CD45RO⁺) CD8⁺ T-cell subsets in leukapheresis samples from 11 healthy adult donors (*P < .01, **P < .001). (B) Representative flow cytometric dot plot with overlay of FACS-sorted cells from the T_N, T_CM, and T_EM subsets. The purity of each subset is indicated in parentheses. The dot plot is on log axes.