A blocking anti-CD47 antibody inhibits dissemination of Raji cells to multiple major organs. Mice engrafted subcutaneously with Raji cells and treated with either IgG control (A) or anti-CD47 antibody (B; from Figure 2) were killed after 1 month, and major organs were analyzed for dissemination of human lymphoma by H&E staining. Lymphoma dissemination was observed in several major organs of IgG control-treated mice as demonstrated by invasion of monomorphic lymphoma cells and disruption of normal cellular architecture. (A) Red asterisks indicate areas of lymphoma invasion. (B) No evidence of human lymphoma was observed in anti-CD47 antibody-treated mice. (C) Human lymphoma organ invasion was assessed by a blinded pathologist. The number of mice with disseminated lymphoma in each organ is indicated.