Grp78 heterozygosity impedes PTEN null-induced leukemogenesis. (A-B) Representative PCR genotyping results from WT (P^f/f78^f/+), Pten null (cP^f/f) and Pten null, Grp78 heterozygous mice (cP^f/f78^f/+) BM (A) and splenocytes (B) 6 days after completion of pIpC treatment. (C-D) Western blot results for detection of GRP78 and PTEN protein level in the BM (C) and splenocytes (D) performed in duplicates. (E) Top panel: Hunched posture of PTEN null mice compared with normal posture of WT and cP^f/f78^f/+ mice. Bottom panel: Detection of immature progenitor cells (dark arrows) in the blood smear of PTEN null mice. A magnified image of one of these cells is shown in the upper right corner. Blood smears were mounted with Pro-Texx mounting medium (Lerner Laboratories, #137635). Images were acquired with a Leica Type DMLB2 microscope (63×/1.4 oil objective) fitted with SPOT RT KE SE Model 7.3 Three Shot Color camera and SPOT 4.0.5 computer software (Diagnostic Instruments Inc). Scale bar represents 30 μm.