Erythroid differentiation of SCD iPSC clones. (A) In vitro hematopoietic differentiation of various iPSC clones to generate HBB-expressing erythroid cells. After 14 days of EB-mediated spontaneous differentiation, iPSC-derived cells were further differentiated and expanded into immature erythroid cells (erythroblasts or EryB) for another 8 days. Then, the erythroid cells were collected for flow cytometry, Giemsa stain of cytospin, and RT-PCR or qRT-PCR. (B) Flow cytometric analysis of S1, cre4, and cre16 using erythroid-specific surface markers CD71 and CD235a (glycophorin A). (C) Giemsa stain of confirming that most of differentiated cells are erythroblasts.