Gal-9 is expressed on C1498FFDsR cells and immune cells. C1498FFDsR cells were untreated or treated with IFN-γ (1000 ng/mL) for 48 hours. Gal-9 expression was assessed by flow cytometry as described. (A) C1498FFDsR cells express gal-9 (> 90%). IFN-γ treatment (1000 U/mL, 48 hours) did not alter the expression of gal-9 on C1498FFDsR cells. B6 mice were injected intravenously with 10⁶ C1498FFDsR. (B-C) Leukocytes from the liver, BM, and spleen of either naive or AML-bearing B6 mice (4 mice/group, 25 days post-AML injection) were prepared. Gal-9 expression was determined by flow cytometry. (B) Flow histogram graph of gal-9 expression on CD11b⁺Gr-1⁺, CD11c⁺, and CD11b⁺Gr-1⁻ cells from the liver, BM, and spleen are shown. Gal-9 is expressed on all 3 types of cells in various organs measured (blue). The presence of AML (red) did not change the pattern of gal-9 expression (blue) in all 3 organs. (C) Bar graphs of the total number of gal-9 expressing CD11b⁺Gr-1⁺, CD11c⁺, and CD11b⁺Gr-1⁻ cells in the liver, BM, and spleen from naive or AML-bearing mice are shown.