Effect of the CXCR1/2 antagonist SCH527123 on TEER of HPAEC monolayers transduced with BMPR-IIsh, NTCsh, or an EV. Confirmation of efficient knockdown of BMPR-II using lentivirus transduction of HPAECs transduced with BMPR-II shRNA, NTC shRNA, or an EV was confirmed at the RNA and protein level (A). HPAECs were then seeded into wells containing 40 gold electrodes and cultured for 24 hours. Capacitance (B,E) and TEER (C-D,F) were measured over a 2-hour period. Then vehicle (C,F) or the CXCR1/2 antagonist SCH527123 (10nM) (D,F) was added to each well and TEER measured for a further 2 hours. (B-D) Representative traces of capacitance and TEER before the addition of vehicle (B-C) and after the addition of SCH527123 (D). (E-F) Data are the mean ± SEM for a minimum 6 experiments. (A) One-way ANOVA showed a significant effect of transduction with shRNA targeting BMPR-II. ***P < .001, compared with EV and NTC shRNA-transduced cells by Tukey test. (B) One-way ANOVA showed a significant effect of transduction with shRNA targeting BMPR-II. *P < .05, **P < .01, and ***P < .001, compared with EV and NTC shRNA-transduced cells by Tukey test.