Neutrophils released by plerixafor function normally. (A) ROS production. DHR was loaded into isolated neutrophils, which were then stimulated with PMA. Data are from a single experiment in which purified blood neutrophils from patient 2 were used, gated as shown in orange on the left and displayed as a histogram on the right. Data are representative of 2 independent experiments, one each for patients 2 and 3, each tested on day +4 of the protocol (Figure 1). Neutrophils were obtained 3 hours after the administration of 0.24 mg/kg plerixafor. FSC indicates forward scatter; SSC, side scatter. (B) Staphylocidal assay. S aureus was incubated at either 2 or 8 bacteria/neutrophil for the time indicated on the x-axis in vitro at 37°C. Cells were then lysed and plated on agar to determine the viable bacterial count. Data are from a single experiment using neutrophils from patient 2 and a single healthy donor (normal) incubated at 2 bacteria/cell and are representative of 4 experiments performed, one for patients 2 and 3 at the 2 concentrations of bacteria. Patient neutrophils were obtained on day +4 of the protocol, 3 hours after the administration of 0.24 mg/kg plerixafor (Figure 1). Control, S aureus incubated without neutrophils.