NK-cell degranulation and IFN-γ secretion in response to TB40/E-infected moDCs involves NKp46 and DNAM-1 activating receptors. (A) NK-cell degranulation against moDCs (moDCs/NK = 1:4) was measured by the CD107a mobilization assay in the presence of blocking mAbs as described in “NK-cell functional assays.” Assays were performed 48 hours after DC exposure to the virus. For each experiment, data were normalized to the response of NK cells incubated with HCMV-infected moDCs in the absence of mAbs (100%); in these conditions, the numbers of CD107a⁺ cells ranged from 11.4% to 18.8%. (B) The same experimental conditions used for degranulation assays were applied. At 6 hours, supernatants were harvested and assayed for the presence of IFN-γ by ELISA. Data were normalized to the IFN-γ levels detected in supernatants of NK cells incubated with TB40/E moDCs in the absence of mAbs (100%); in these conditions, the absolute concentrations of IFN-γ ranged from 164 to 915 pg/mL. Data are mean plus or minus SEM. *P < .05.