Specific NK-cell activation in response to autologous HCMV-infected moDCs. (A-B) Freshly purified NK cells were cultured for 48 hours alone, or with mock moDCs, UV-TB40/E-treated moDCs, or TB40/E-infected moDCs in the presence of 10 U/mL of IL-2 (moDCs/NK = 1:20). (A) CD25 and CD69 expression was analyzed by flow cytometry. (B) IFN-γ production was detected in culture supernatants by ELISA. Results from 3 representative experiments performed with different donors are shown. Numbers correspond to mean value of IFN-γ production. (C-D) NK cells purified by negative selection from PBMCs stimulated overnight with IL-2 were cocultured for 5 hours with target cells as described in “NK-cell functional assays.” Surface CD107a expression in CD56⁺ cells was analyzed by flow cytometry. (C) Dot plots from a representative experiment of 8 performed are shown (moDCs/NK = 1:4), including the K562 cell line as a control. The percentage of CD107a⁺ cells is included in each dot plot (TB40/E moDCs: 65% 1E-1/IE-2⁺cells) (D) Scatter plots displaying the percentage of CD107a⁺ cells from 8 different experiments performed.