c-Fos expression is silenced during DC maturation. (A) Time-course RT-PCR experiments were performed to quantify the expression of c-Fos mRNA and miR155 in mouse DC²¹¹⁴ cells stimulated with CpG + αCD40 and human Mo-DCs stimulated with LPS or poly (I:C). Results are represented as the fold change in the expression of c-Fos mRNA (left axes) and miR155 (right axes). Representative experiments are shown for the top 2 panels. The means and SDs derived from 2 experiments are shown for the bottom panel. (B) c-Fos mRNA was quantified by real-time RT-PCR in unstimulated and LPS-treated CD8α⁺ and CD8α⁻ splenic DCs. Results are represented as the fold change in c-Fos mRNA expression. The means and SDs derived from 2 independent experiments are shown. (C) c-Fos expression was analyzed in microarray data derived from BM-pDCs stimulated for 0, 4, and 24 hours with imiquimod. Results are represented as the fold change in signal intensities for c-Fos mRNA. The means and SDs derived from 3 experiments are shown. (D) c-Fos mRNA was quantified by real-time RT-PCR in unstimulated mouse BM-DCs and BM-DCs stimulated with LPS, poly (I:C), PGN, PAM3CSK4, FSL-1, imiquimod, CpG, or MDP. Results are represented as the fold change in c-Fos mRNA expression. The means and SDs derived from 2 independent experiments are shown.