NHL monocytes have impaired response to stimuli. (A) Whole blood was stimulated as indicated (parentheses on left axis) and immediately processed for analysis by flow cytometry of monocyte intracellular STAT protein phosphorylation. Columns represent mean plus or minus SD (CpG stimulation: controls, n = 3; lymphoma, n = 10; IFN-γ and IL-6 stimulation: control, n = 5; lymphoma, n = 7). Representative histograms for each condition are shown: middle panel, control sample; right panel, lymphoma sample (shaded area represents isotype control; black line, sample protein phosphorylation). (B) PBMCs from control and lymphoma patients were incubated overnight with either poly I:C (left axis, n = 4) or CpG (right axis, n = 4) and assessed for IFN-α concentration in culture supernatant by ELISA. (C) Monocytes from control and lymphoma patients were isolated with anti-CD14 immunomagnetic beads and matured with TNF-α and PGE2. Bars represent mean for each group. Statistically significant P values are shown between groups.
