Figure 4
Figure 4. Flow cytometric analysis of MKs infected with control and stathmin-expressing lentiviruses. (A) The density plot on the left represents uninfected MKs that were used as negative control for GFP expression. The density plot in the middle represents infected MKs showing GFP expression (x-axis). The dot plot on the right represents GFP+ MKs that express CD41 (x-axis) and CD42b (y-axis). (B) Quantification of CD41+/CD42+ MKs infected with control (GFP), WT, or nonphosphorylatable mutant (4A) stathmin-expressing lentiviruses. The results are represented as fold change in the percentage of CD41+/CD42b+ MKs in 3 independent experiments ± SD.

Flow cytometric analysis of MKs infected with control and stathmin-expressing lentiviruses. (A) The density plot on the left represents uninfected MKs that were used as negative control for GFP expression. The density plot in the middle represents infected MKs showing GFP expression (x-axis). The dot plot on the right represents GFP+ MKs that express CD41 (x-axis) and CD42b (y-axis). (B) Quantification of CD41+/CD42+ MKs infected with control (GFP), WT, or nonphosphorylatable mutant (4A) stathmin-expressing lentiviruses. The results are represented as fold change in the percentage of CD41+/CD42b+ MKs in 3 independent experiments ± SD.

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