Electron micrographs of migrating neutrophils in WT and LSP1^−/− mice. (A) WT; (B) Lsp1^−/− mice. Sections (70 nm) were taken after KC superfusion (5nM) of the cremaster. Images and corresponding cartoons demonstrate endothelial dome formations (arrows) in WT mice (A) and the lack of such formations in LSP1 deficient mice (B arrow). Scale bars indicate 2 μm (A) and 1 μm (B). Image acquisition, panels A-B: Hitachi H-7000 transmission electron microscope; direct magnification: 3000× (A), 4000× (B); 16000 AMT camera, AMT Capture Engine software (V.600.128); images generated with Microsoft Office PowerPoint 2003 (SP3). Percentage quantification of the electron micrograph sections based on the adherent neutrophils per vessel that underwent transendothelial migration in WT vs Lsp1^−/− animals (C). Thirty-eight to 42 vessel sections per background were analyzed. ***P < .001. Error bars indicate SEM. e1-e3 indicate endothelial cells; n, neutrophil; and L, vessel lumen.